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pesc- ura3 vector under inducible gal1 promoter  (GenScript corporation)

 
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    GenScript corporation pesc- ura3 vector under inducible gal1 promoter
    Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and <t>pESC-</t> <t>URA3</t> vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.
    Pesc Ura3 Vector Under Inducible Gal1 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pesc- ura3 vector under inducible gal1 promoter/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    pesc- ura3 vector under inducible gal1 promoter - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Evidence for a Conserved Function of Eukaryotic Pantothenate Kinases in the Regulation of Mitochondrial Homeostasis and Oxidative Stress"

    Article Title: Evidence for a Conserved Function of Eukaryotic Pantothenate Kinases in the Regulation of Mitochondrial Homeostasis and Oxidative Stress

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24010435

    Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and pESC- URA3 vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.
    Figure Legend Snippet: Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and pESC- URA3 vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.

    Techniques Used: Mutagenesis, Plasmid Preparation, Incubation

    Analysis of reactive oxygen species (ROS) yeast PanK-deficient mutants expressing human PanK isoforms. ROS measurements were performed for cab1 Δ/ cab1 G351S ( A ), cab1 Δ/ cab1 N290I ( C ), and cab1 Δ/ cab1 I291T ( E ) yeast strains transformed with pFL38/ CAB1 , EV, and pESC- URA3 vector containing different human PanK isoforms. ROS production was determined as the percentage of fluorescent cells using the DHR123 probe. Cytofluorometry analysis of the cab1 Δ/ cab1 G351S ( B ), cab1 Δ/ cab1 N290I ( D ), and cab1 Δ/ cab1 I291T ( F ) yeast strains transformed with either pFL38/ CAB1 , empty vector or pESC- URA3 vector containing different human PanK isoforms following staining with DHR123 probe. Each curve represents the distribution of the measured events (counts) according to their fluorescence intensity expressed in a log unit. The grey spectrum represents background autofluorescence. The strains were grown in YP medium supplemented with 2% galactose at 28 °C. This assay was conducted in triplicate ( n = 3) and the plotted graphs represent the average ± SEM. *: p < 0.05 and **: p < 0.01 relative to mutant strain transformed with CAB1 allele and #: p < 0.05 and ##: p < 0.01 relative to mutant strain transformed with the empty vector.
    Figure Legend Snippet: Analysis of reactive oxygen species (ROS) yeast PanK-deficient mutants expressing human PanK isoforms. ROS measurements were performed for cab1 Δ/ cab1 G351S ( A ), cab1 Δ/ cab1 N290I ( C ), and cab1 Δ/ cab1 I291T ( E ) yeast strains transformed with pFL38/ CAB1 , EV, and pESC- URA3 vector containing different human PanK isoforms. ROS production was determined as the percentage of fluorescent cells using the DHR123 probe. Cytofluorometry analysis of the cab1 Δ/ cab1 G351S ( B ), cab1 Δ/ cab1 N290I ( D ), and cab1 Δ/ cab1 I291T ( F ) yeast strains transformed with either pFL38/ CAB1 , empty vector or pESC- URA3 vector containing different human PanK isoforms following staining with DHR123 probe. Each curve represents the distribution of the measured events (counts) according to their fluorescence intensity expressed in a log unit. The grey spectrum represents background autofluorescence. The strains were grown in YP medium supplemented with 2% galactose at 28 °C. This assay was conducted in triplicate ( n = 3) and the plotted graphs represent the average ± SEM. *: p < 0.05 and **: p < 0.01 relative to mutant strain transformed with CAB1 allele and #: p < 0.05 and ##: p < 0.01 relative to mutant strain transformed with the empty vector.

    Techniques Used: Expressing, Transformation Assay, Plasmid Preparation, Staining, Fluorescence, Mutagenesis



    Similar Products

    pesc- ura3 vector under inducible gal1 promoter  (GenScript corporation)
    90
    GenScript corporation pesc- ura3 vector under inducible gal1 promoter
    Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and <t>pESC-</t> <t>URA3</t> vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.
    Pesc Ura3 Vector Under Inducible Gal1 Promoter, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pesc- ura3 vector under inducible gal1 promoter/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    pesc- ura3 vector under inducible gal1 promoter - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and pESC- URA3 vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence for a Conserved Function of Eukaryotic Pantothenate Kinases in the Regulation of Mitochondrial Homeostasis and Oxidative Stress

    doi: 10.3390/ijms24010435

    Figure Lengend Snippet: Complementation of the thermosensitive phenotype of CAB1 -defective yeast strains by human PanKs. ( A – C ) Equal amounts of serial dilutions of cells (10 4 , 10 3 , 10 2 , 10 1 cells/spot) from exponentially grown cultures of cab1 Δ/ cab1 G351 S ( A ), cab1 Δ/ cab1 N290I ( B ), and cab1 Δ/ cab1 I291T ( C ) mutant strains carrying pFL38/ CAB1 and pESC- URA3 vector containing the different h PANK gene were spotted onto SC medium supplemented with the indicated carbon sources and incubated at 28 °C and 37 °C. Yeast growth was monitored over time and images collected 2 or 3 days post-inoculation. ( D – F ) Growth rates of cab1 Δ/ cab1 G351S ( D ), cab1 Δ/ cab1 N290I ( E ), and cab1 Δ/ cab1 I291T ( F ) strains carrying the empty vector (EV), CAB1 and human PANK s in liquid media on glucose or galactose at 30 °C vs. 37 °C (from top to bottom). Yeast cells were inoculated in minimal medium without uracil and tryptophan but containing either 2% glucose or 2% galactose at a cell density of 10 4 cells/mL. Optical density measurements at 600 nm (OD 600 ) were determined at the indicated time points. Data for liquid assays represent three biological replicates ( n = 3) and the plotted graphs represent the average ± SEM.

    Article Snippet: The human PANK genes, PANK1 (Uniprot #Q8TE04), PANK2 (Uniprot # Q9BZ23), and PANK3 (Uniprot # Q9H999) were cloned into the pESC- URA3 vector under the inducible GAL1 promoter (GenScript Inc., Piscataway, NJ, United States), which results in expression of the proteins with an N-terminal Myc tag.

    Techniques: Mutagenesis, Plasmid Preparation, Incubation

    Analysis of reactive oxygen species (ROS) yeast PanK-deficient mutants expressing human PanK isoforms. ROS measurements were performed for cab1 Δ/ cab1 G351S ( A ), cab1 Δ/ cab1 N290I ( C ), and cab1 Δ/ cab1 I291T ( E ) yeast strains transformed with pFL38/ CAB1 , EV, and pESC- URA3 vector containing different human PanK isoforms. ROS production was determined as the percentage of fluorescent cells using the DHR123 probe. Cytofluorometry analysis of the cab1 Δ/ cab1 G351S ( B ), cab1 Δ/ cab1 N290I ( D ), and cab1 Δ/ cab1 I291T ( F ) yeast strains transformed with either pFL38/ CAB1 , empty vector or pESC- URA3 vector containing different human PanK isoforms following staining with DHR123 probe. Each curve represents the distribution of the measured events (counts) according to their fluorescence intensity expressed in a log unit. The grey spectrum represents background autofluorescence. The strains were grown in YP medium supplemented with 2% galactose at 28 °C. This assay was conducted in triplicate ( n = 3) and the plotted graphs represent the average ± SEM. *: p < 0.05 and **: p < 0.01 relative to mutant strain transformed with CAB1 allele and #: p < 0.05 and ##: p < 0.01 relative to mutant strain transformed with the empty vector.

    Journal: International Journal of Molecular Sciences

    Article Title: Evidence for a Conserved Function of Eukaryotic Pantothenate Kinases in the Regulation of Mitochondrial Homeostasis and Oxidative Stress

    doi: 10.3390/ijms24010435

    Figure Lengend Snippet: Analysis of reactive oxygen species (ROS) yeast PanK-deficient mutants expressing human PanK isoforms. ROS measurements were performed for cab1 Δ/ cab1 G351S ( A ), cab1 Δ/ cab1 N290I ( C ), and cab1 Δ/ cab1 I291T ( E ) yeast strains transformed with pFL38/ CAB1 , EV, and pESC- URA3 vector containing different human PanK isoforms. ROS production was determined as the percentage of fluorescent cells using the DHR123 probe. Cytofluorometry analysis of the cab1 Δ/ cab1 G351S ( B ), cab1 Δ/ cab1 N290I ( D ), and cab1 Δ/ cab1 I291T ( F ) yeast strains transformed with either pFL38/ CAB1 , empty vector or pESC- URA3 vector containing different human PanK isoforms following staining with DHR123 probe. Each curve represents the distribution of the measured events (counts) according to their fluorescence intensity expressed in a log unit. The grey spectrum represents background autofluorescence. The strains were grown in YP medium supplemented with 2% galactose at 28 °C. This assay was conducted in triplicate ( n = 3) and the plotted graphs represent the average ± SEM. *: p < 0.05 and **: p < 0.01 relative to mutant strain transformed with CAB1 allele and #: p < 0.05 and ##: p < 0.01 relative to mutant strain transformed with the empty vector.

    Article Snippet: The human PANK genes, PANK1 (Uniprot #Q8TE04), PANK2 (Uniprot # Q9BZ23), and PANK3 (Uniprot # Q9H999) were cloned into the pESC- URA3 vector under the inducible GAL1 promoter (GenScript Inc., Piscataway, NJ, United States), which results in expression of the proteins with an N-terminal Myc tag.

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Staining, Fluorescence, Mutagenesis